Principles of recombinant production

Recombinant proteins are often used as medicines or for their ability to identify other materials (proteins, viruses, bacteria and so on). To produce a recombinant protein, one needs the description and a factory. Genes are the descriptions for proteins, and different cells can be used as factories.

General steps:

  1. Find the genetic code for the protein to be produced, cut or copy from origin
  2. Insert the gene into a cell (safe bacteria, insect cell, plant cell, animal cell or human cell)
  3. With the new gene inside, the cell will produce the protein described by this gene (along with other proteins described by the other original genes)
  4. Cells are usually grown in stirred tanks, bioreactors, which provides suitable environment with enough nutrients. This allows one cell to divide into two identical copies, which in turn divides further.
  5. The protein is harvested from the cell soup and purified so that all other proteins and cell products are removed