Principles of recombinant production
Recombinant proteins are often used as medicines or for their ability to identify other materials (proteins, viruses, bacteria and so on). To produce a recombinant protein, one needs the description and a factory. Genes are the descriptions for proteins, and different cells can be used as factories.
- Find the genetic code for the protein to be produced, cut or copy from origin
- Insert the gene into a cell (safe bacteria, insect cell, plant cell, animal cell or human cell)
- With the new gene inside, the cell will produce the protein described by this gene (along with other proteins described by the other original genes)
- Cells are usually grown in stirred tanks, bioreactors, which provides suitable environment with enough nutrients. This allows one cell to divide into two identical copies, which in turn divides further.
- The protein is harvested from the cell soup and purified so that all other proteins and cell products are removed